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| Registros recuperados: 73 | |
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| Le Moullac, Gilles; Phillip De Laborie, Loic; Saulnier, Denis; Goarant, Cyrille; Dehasque, Marleen. |
| Anderson (1992) y Raa (1996) han escrito impresionantes críticas acerca del uso de inmunoestimulantes en acuacultura. La mayoría de los trabajos citados se refieren principalmente al cultivo de peces y pocos acerca del camarón. Así que este trabajo está enfocado al camarón y las pocas pruebas publicadas. Varios de los inmunoestimulantes y su manera de actuar sobre el sistema inmune en camarones se describen brevemente, así como los métodos usados para administrar las sustancias inmunoestimulatorias para camarón. La dificultad para experimentar en este campo está señalada con algunos ejemplos. |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00029/14011/11197.pdf |
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| Aguirre-guzman, Gabriel; Labreuche, Yannick; Ansquer, Dominique; Espiau, Benoit; Levy, Peva; Ascencio, Felipe; Saulnier, Denis. |
| The pathogenicity of two V. penaeicida strains, AM101 and KH-1, with different geographic origin, and V. nigripulchritudo strain AM102, were investigated in juvenile blue shrimp species Litopenaeus stylirostris. Alive bacteria and protein fractions (PFs) obtained from cell-free supernatants (CFS) were used in experimental challenges. Strains AM102, AM101, and KH-1 produced respectively 60, 54 and 12% mortality at 96h after infection using 104 cfu mL-1 of bacterial suspension. Exocellular toxin-like factors were evidenced in CFS from the New Caledonian strains (AM102 and AM101) but not the Japanese strain (KH-1). At 48h post injection of each CFS, mortality rates were respectively 96,98 and 5% when these strains were cultivated at 20°C, whereas only O,16... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus stylirostris; Vibrio nigripulchritudo; Vibrio penaeicida. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2807.pdf |
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| Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel. |
| The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder... |
| Tipo: Text |
Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf |
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| Cochennec-laureau, Nathalie; Levy, Peva; Saulnier, Denis; Fougerouse, Angelique. |
| L’importance socio-économique de l’activité perlière justifie que le Service de la Perliculture mette en place un réseau de surveillance pathologique de l’huître perlière Pinctada margaritifera en Polynésie française. Les objectifs de ce réseau sont de prévenir l’introduction ou l’apparition d’agents infectieux, de prévenir leur propagation à l’intérieur d’une zone d’élevage, d’étudier les moyens de limiter leur impact, de surveiller leur évolution et enfin de garantir les échanges entre les zones d’élevage. Cette première année a permis d’élaborer le schéma de fonctionnement du réseau et de vérifier sa faisabilité. Cinq zones de prélèvements ont été définies en fonction de critères d’accessibilité à la zone et de facilité d’acheminement des échantillons,... |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00168/27886/26141.pdf |
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| Faury, Nicole; Saulnier, Denis; Thompson, F; Gay, Melanie; Swings, J; Leroux, Frederique. |
| Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T) =LMG 22240(T)=Clp 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine... |
| Tipo: Text |
Palavras-chave: Identification; Vibrio; Crasssotrea gigas; Ostreiculture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-591.pdf |
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| Aguirre Guzman, G; Ascencio, F; Saulnier, Denis. |
| The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus vannamei; Cysteine protease; Vibrio penaeicida. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3618.pdf |
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| Le Roux, Frederique; Goubet, A; Thompson, F; Faury, Nicole; Gay, Melanie; Swings, J; Saulnier, Denis. |
| Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase Quo factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulatory protein (toxR) genes, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that... |
| Tipo: Text |
Palavras-chave: Vibrio gigantis; Haemoplymph; Disease; Crassostrea gigas; Oyster culture. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-792.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique. |
| A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp... |
| Tipo: Text |
Palavras-chave: Virulence; Epidemiology; Typing; MLST; AP PCR; Mariculture; Vibriosis. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1913.pdf |
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| Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis. |
| In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the... |
| Tipo: Text |
Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf |
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| Nicolas, Jean-louis; Renault, Tristan; Gagnaire, Beatrice; Garcia, Celine; Le Roux, F; Miossec, Laurence; Pepin, Jean-francois; Saulnier, Denis. |
| L'un des objectifs principaux des travaux en pathologie des mollusques est d'identifier et d'étudier des agents infectieux capables d'induire des maladies et des mortalités chez les coquillages, en particulier, chez I'huître creuse, Crassostrea gigas, espèce principalement exploitée en France. Ces travaux semblent d'autant plus indispensables qu'il n'existait pas, jusqu'à ces dernières années, de maladies identifiées chez I'huître creuse en Europe excepté le virus herpès suspecté dès 1995 de provoquer des mortalités de naissain (Renault et al, 1995). Les principales maladies des espèces proches sont dues à des parasites comme Haplosporidium nelsoni (Ford et Heskin, 1987) et Perkinsus marinus Olivier et al, 1 998) pour Crassostrea virginica, Marteilia... |
| Tipo: Text |
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| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00304/41535/40743.pdf |
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| Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra. |
| In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF |
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| De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis. |
| Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and... |
| Tipo: Text |
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf |
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| Arnaud, Sophie; Goyard, Emmanuel; Vonau, Vincent; Herbaut, C; Prou, Jean; Saulnier, Denis. |
| Most bivalves species of the genus Pinctada are well known throughout the world for production of white or black pearls of high commercial value. For cultured pearl production, a mantle allograft from a donor is implanted into the gonad of a recipient oyster, together with a small inorganic bead. Because of the dedifferentiation of cells during the first steps of the host oyster's immunological reaction, so far the fate of the graft and its exact role in the process of pearl formation could not be determined via classical histological methods. Here we report the first molecular evidence of the resilience of the graft in the recipient organism by showing that cells containing genome from the donor are still present at the end of pearl formation. These... |
| Tipo: Text |
Palavras-chave: Genotype; Pearl; Pearl sac; Grafting; Biomineralization; Pearl oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2456.pdf |
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| Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis. |
| Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus.. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf |
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| Lopez Flores, L; Suarez Santiago, V; Longet, D; Saulnier, Denis; Chollet, Bruno; Arzul, Isabelle. |
| Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and... |
| Tipo: Text |
Palavras-chave: Molecular phylogeny; Actin gene; Ostrea edulis parasite; Bonamia ostreae. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3562.pdf |
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| Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan. |
| To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR).... |
| Tipo: Text |
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf |
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| Leroux, Frederique; Binesse, Johan; Saulnier, Denis; Mazel, Didier. |
| splendidus is a dominant culturable Vibrio in seawater, but strains related to this group are also associated with mortalities in a variety of marine animals. The determinants encoding the pathogenic properties of these strains are still poorly known, however, the recent sequencing of the genome of V. splendidus LGP32, an oyster pathogen, offers the opportunity to decipher the basis of the virulence properties by the disruption of candidate genes. We have developed a novel suicide vector based on the pir-dependant R6K replicative origin, which can be transferred by RP4 based conjugation to, potentially, any Vibrio strain, and which also carries the plasmid F toxin ccdB gene under the control of the PBAD promoter. We demonstrate that this genetic system... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3292.pdf |
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| Registros recuperados: 73 | |
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